This book requires the basic knowledge of human physiology, cell biology, Molecular Biology, Animal biotechnology and Biochemistry. The goal of this book is to provide students, teachers and researchers the essence of the key concepts and deep knowledge of stem cells basics, stem cell technology and their application in field of regenerative medicine applying these thematic studies of stem cell technology and tissue engineering. Stem cell technology and it’s application in Tissue engineering is an interdisciplinary field of biomedical research compiling Biosciences, material sciences and Bioengineering to progress the treatment of damaged, degenerated and diseased tissue through maintenance, repair, and replacement methodology. This ever-emerging area of research applies in numerous medical and interdisciplinary streams of stem cell biology, tissue regeneration, regenerative medicine and Bioengineering. These stem cell biology techniques and tissue engineering strategies are now being progressively more evaluated and progressed as potential treatments of neuro degeneration disorders, regenerative medicine, artificial organ development, organ bioengineering, and transplant medicine. The book seeks to put an end to many queries and doubts about an understanding of the clinical need for stem cell therapy and tissue engineering in regenerative medicine, knowledge of stem cell sources and their isolation, characterization, principle of tissue engineering using basic elements and concept of tissue engineering. Further, the need for biomaterials, their design, fabrication and used of the biomaterials for in vitro, in vivo and clinical application are also covered. This book discusses the emergence of stem cell technology, tissue engineering and our current understanding of the Biotechnology and Biological sciences discipline